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Asthma Sufferers and Their Caregivers Are Asked to Help Design a Medical …
Fort Mills Times A market research firm, Consilium Associates, is conducting an online survey on behalf of a medical technology company that is developing a medical device that will predict when someone with asthma will have an attack. The medical technology company is … |
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Comprehensive FISH Probe Design Tool Applied to Imaging Human Immunoglobulin Class Switch Recombination.
PLoS One. 2012;7(12):e51675
Authors: Nedbal J, Hobson PS, Fear DJ, Heintzmann R, Gould HJ
Abstract
We present a web engine boosted fluorescence in-situ hybridization (webFISH) algorithm using a genome-wide sequence similarity search to design target-specific single-copy and repetitive DNA FISH probes. The webFISH algorithm featuring a user-friendly interface (http://www.webfish2.org/) maximizes the coverage of the examined sequences with FISH probes by considering locally repetitive sequences absent from the remainder of the genome. The highly repetitive human immunoglobulin heavy chain sequence was analyzed using webFISH to design three sets of FISH probes. These allowed direct simultaneous detection of class switch recombination in both immunoglobulin-heavy chain alleles in single cells from a population of cultured primary B cells. It directly demonstrated asynchrony of the class switch recombination in the two alleles in structurally preserved nuclei while permitting parallel readout of protein expression by immunofluorescence staining. This novel technique offers the possibility of gaining unprecedented insight into the molecular mechanisms involved in class switch recombination.
PMID: 23272136 [PubMed – in process]
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Healthy Foods Help Ease Asthma Symptoms
Food Product Design TAIPEI, Taiwan—Probiotics, fish oil, and fruit and vegetable concentrate may help ease respiratory problems in asthmatic children, according to a new study published in the British Journal of Nutrition. Observational studies have found negative … |
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Starhaler® Device Finalist at Medical Design Excellence Awards
MarketWatch (press release) MUMBAI, India, April 10, 2012 /PRNewswire via COMTEX/ — SPARC Ltd's Combitide Starhaler® for asthma and COPD is a finalist at the 2012 Medical Design Excellence Awards (MDEA) to be held in Philadelphia this May. MDEA is the premier awards program for … |
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Raw Milk May Reduce Kids' Allergies, Asthma
Food Product Design BASEL, Switzerland—A new study linking raw milk to a reduced incidence of allergies and asthma among children adds more fuel to the raw milk debate that has been occurring over the past few years. The study, published in the Journal of Allergy and … |
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How Design Cures Chest Pains, Sticker Shock and Asthma
The Faster Times To think about our most piercing challenges, consider a fact I've heard three years running that still shocks me: more New Yorkers die from chronic diseases … |
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