New approaches to qualitative interviewing: Development of a card sort technique to understand subjective patterns of symptoms and responses.

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New approaches to qualitative interviewing: Development of a card sort technique to understand subjective patterns of symptoms and responses.

Int J Nurs Stud. 2016 Jan 9;

Authors: Mammen JR, Norton SA, Rhee H, Butz AM

Abstract
BACKGROUND: Ability to elicit individuals’ perceptions of complex behavioral processes can be challenging, as it hinges not only upon the skill of the researcher, but also upon assumptions of a shared language and individuals’ ability to recall, analyze, and effectively communicate events. In a case-based qualitative-descriptive study about teens’ experiences of asthma self-management, we found that variations in terminology and descriptions of events, symptoms, and responses made it difficult to understand teens’ experiences of asthma. In particular, teens’ conceptualization of their asthma symptoms and self-management responses differed from situation to situation, from other teens in the study, from the interviewer, and from prior reports in the literature. These differences across many levels made it difficult to identify patterns in individual processes of self-management, and among teens in general..
OBJECTIVES: To address these challenges, we developed a card sorting activity to facilitate in-depth exploration of teens’ experiences of asthma.
DESIGN: Case-based qualitative description.
SETTING: Teen-parent dyads (N=28) were recruited from the community, Emergency Department, Pediatric Pulmonary Department, and prior study subjects of a major medical center.
METHODS: Teens first identified and then sequenced their own unique sets of asthma symptoms and self-management responses. Teens then developed contextually grounded narratives using the card sort they had created as a visual aid.
RESULTS: This technique not only allowed us to bridge teen-interviewer communication barriers and develop shared terminology, but also resulted in a visible sequence of asthma symptoms and self-management responses.
CONCLUSIONS: The card sort technique facilitated researcher-teen discussion and enabled comparison of self-management patterns across teens in our study. This technique is potentially useful for other areas of research exploring behavioral processes with complex and individual-specific experiences, in particular those involving sequences of events and self-management responses. This paper delineates the development, utility, and potential applications of the symptom-response card sorting technique for research and clinical practice.

PMID: 26897540 [PubMed – as supplied by publisher]

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Role of Local Eosinophilopoietic Processes in the Development of Airway Eosinophilia in Prednisone-dependent Severe Asthma.

Role of Local Eosinophilopoietic Processes in the Development of Airway Eosinophilia in Prednisone-dependent Severe Asthma.

Clin Exp Allergy. 2015 Dec 18;

Authors: Sehmi R, Smith SG, Kjarsgaard M, Radford K, Boulet LP, Lemiere C, Prazma CM, Ortega H, Martin JG, Nair P

Abstract
RATIONALE: In severe asthmatics with persistent airway eosinophilia, blockade of the eosinophilopoietin, interleukin-5 has significant steroid sparing effects and attenuates blood and sputum eosinophilia. The contribution of local maturational processes of progenitors within the airways relative to the recruitment of mature cells from the peripheral circulation to airway eosinophilia in these patients is not known. We hypothesize that local eosinophilopoietic processes may be the predominant process that drives persistent airway eosinophilia and corticosteroid requirement in severe asthmatics.
METHODS: In a cross-sectional study, the number and growth potential of eosinophil-lineage committed progenitors (EoP) were assayed in 21 severe eosinophilic asthmatics, 19 mild asthmatics, 8 COPD patients and 8 normal subjects. The effect of anti-IL-5 treatment on mature eosinophils and EoP numbers was made in severe eosinophilic asthmatics who participated in a randomized clinical trial of mepolizumab (sub-study of a larger GSK sponsored global phase III trial, MEA115575) where subjects received mepolizumab (100 mg, n=9) or placebo (n=8), as six monthly subcutaneous injections.
RESULTS: Mature eosinophil and EoP numbers were significantly greater in the sputum of severe asthmatics compared with all other subject groups. In colony forming assays, EoP from blood of severe asthmatics demonstrated a greater response to IL-5 than mild asthmatics. Treatment of severe asthmatics with mepolizumab significantly attenuated blood eosinophils and increased EoP. There was however no significant treatment effect on mature eosinophils, sputum EoP numbers or the prednisone maintenance dose.
CONCLUSIONS: Patients with severe eosinophilic asthma have an exaggerated eosinophilopoeitic process in their airways. Treatment with 100 mg sub-cutaneous mepolizumab significantly attenuated systemic differentiation of eosinophils, but did not suppress local airway eosinophil differentiation to mature cells. Targeting IL-5 driven eosinophil differentiation locally within the lung maybe of relevance for optimal control of airway eosinophilia and asthma. This article is protected by copyright. All rights reserved.

PMID: 26685004 [PubMed – as supplied by publisher]

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A systematic review of predictive models for asthma development in children.

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A systematic review of predictive models for asthma development in children.

BMC Med Inform Decis Mak. 2015;15(1):99

Authors: Luo G, Nkoy FL, Stone BL, Schmick D, Johnson MD

Abstract
BACKGROUND: Asthma is the most common pediatric chronic disease affecting 9.6 % of American children. Delay in asthma diagnosis is prevalent, resulting in suboptimal asthma management. To help avoid delay in asthma diagnosis and advance asthma prevention research, researchers have proposed various models to predict asthma development in children. This paper reviews these models.
METHODS: A systematic review was conducted through searching in PubMed, EMBASE, CINAHL, Scopus, the Cochrane Library, the ACM Digital Library, IEEE Xplore, and OpenGrey up to June 3, 2015. The literature on predictive models for asthma development in children was retrieved, with search results limited to human subjects and children (birth to 18 years). Two independent reviewers screened the literature, performed data extraction, and assessed article quality.
RESULTS: The literature search returned 13,101 references in total. After manual review, 32 of these references were determined to be relevant and are discussed in the paper. We identify several limitations of existing predictive models for asthma development in children, and provide preliminary thoughts on how to address these limitations.
CONCLUSIONS: Existing predictive models for asthma development in children have inadequate accuracy. Efforts to improve these models’ performance are needed, but are limited by a lack of a gold standard for asthma development in children.

PMID: 26615519 [PubMed – in process]

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Relation between sonic hedgehog pathway gene polymorphisms and basal cell carcinoma development in the Polish population.

Relation between sonic hedgehog pathway gene polymorphisms and basal cell carcinoma development in the Polish population.

Arch Dermatol Res. 2015 Nov 21;

Authors: Lesiak A, Sobolewska-Sztychny D, Majak P, Sobjanek M, Wodz K, Sygut KP, Majsterek I, Wozniacka A, Narbutt J

Abstract
In recent decades, increases have been observed in the incidence of nonmelanoma skin cancers, including basal cell carcinoma (BCC) and squamous cell carcinoma. BCC is the most common neoplasm in Caucasian populations. Sonic hedgehog (Shh) pathway impairment plays a key role in BCC pathogenesis, and there is evidence that Shh pathway genetic variations may predispose to BCC development. We genotyped 22 single-nucleotide polymorphisms (SNPs) in 4 Shh pathway genes: SHH, GLI, SMO, and PTCH. The study group consisted of 142 BCC patients and 142 age-matched, sex-matched healthy subjects (controls). SNPs were assessed using the PCR-RFLP method. The genotype distribution for the polymorphisms in the rs104894049 331 A/T SHH, rs104894040 349 T/C SHH, and rs41303402 385 G/A SMO genes differed significantly between the BCC patients and the controls. The presence of CC genotype in the SHH rs104894040 349 T/C polymorphism was linked to the highest risk of BCC development (OR 87.9, p < 0.001). Other genotypes, such as the TT in SHH rs104894049 331 A/T and the GG in SMO rs41303402 385 G/A also statistically raised the risk of BCC, but these associations were weaker. Other investigated polymorphisms showed no statistical differences between patients and controls. The results obtained testify to the importance of the SHH and SMO gene polymorphisms in skin cancerogenesis. These results mainly underline the potential role of SHH3 rs104894040 349 T/C gene polymorphism in the development of skin basal cell carcinomas in patients of Polish origin.

PMID: 26590974 [PubMed – as supplied by publisher]

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Researcher: Blanket Approach to Asthma not Ideal – Drug Discovery & Development


Drug Discovery & Development

Researcher: Blanket Approach to Asthma not Ideal
Drug Discovery & Development
Associate Professor Simon Phipps. Source: University of Queensland University of Queensland research into the effects of dust mite and cockroach allergens has found that different types of asthma respond differently to a new experimental treatment.

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Good gut bacteria might prevent asthma development – ModernMedicine


The Daily Progress

Good gut bacteria might prevent asthma development
ModernMedicine
Therapeutic inoculation with four specific bacteria in infancy could prevent the development of asthma in adulthood, according to a new study from the University of British Columbia (UBC). Researchers found that gut dysbiosis in the first 100 days of
Health: 'Good' bacteria, asthma prevention linkedThe Daily Progress
Gut bacteria deficit linked to higher asthma riskBel Marra Health
Infants with four “good” gut bacteria unlikely to have asthmaLedger Gazette
Latino Post –iFreePress.com (blog) –Latinos Post
all 33 news articles »

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Effect of different helminth extracts on the development of asthma in mice: the influence of early-life exposure and the role of IL-10 response.

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Effect of different helminth extracts on the development of asthma in mice: the influence of early-life exposure and the role of IL-10 response.

Exp Parasitol. 2015 Jun 17;

Authors: Pitrez PM, Gualdi LP, Barbosa GL, Sudbrack S, Ponzi D, Cao RG, Silva AC, Machado DC, Jones MH, Stein RT, Graeff-Teixeira C

Abstract
It is not currently clear whether different parasites have distinct effects on the airway inflammatory response in asthma and whether exposure in early life to helminths have a stronger impact in a potential inhibitory effect on asthma. The aim of this study is to evaluate the effect of exposure to different helminth extracts on the development of allergic pulmonary response in mice, including early-life exposure. Different helminth extracts (Angiostrongylus costaricensis, Angiostrongylus cantonensis and Ascaris lumbricoides) were studied in female adult BALB/c and C57BL/6 IL-10-deficient mice in a protocol of murine asthma, injected intraperitoneally in different periods of exposure (early, pre-sensitization and post-sensitization). Cell counts in bronchoalveolar lavage (BAL), eosinophil peroxidase (EPO) from lung tissue, cytokine levels from BAL/spleen cell cultures, and lung histology were analyzed. Airway cellular influx induced by OVA was significantly inhibited by extracts of A. cantonensis and A. lumbricoides. Extracts of A. lumbricoides and A. costaricensis led to a significant reduction of IL-5 in BAL (p<0.001). Only the exposure to A. lumbricoides led to an increased production of IL-10 in the lungs (p<0.001). In IL-10-deficient mice exposed to A. costaricensis pre-sensitization, eosinophil counts and IL-5 levels in BAL and EPO in lung tissue were significantly reduced. In the early exposure to A. cantonensis, lung inflammation was clearly inhibited. In conclusion, different helminth extracts inhibit allergic lung inflammation in mice. IL-10 may not play a central role in some helminth-host interactions. Early exposure to helminth extracts could be a potential strategy to explore primary prevention in asthma.

PMID: 26093162 [PubMed – as supplied by publisher]

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Atopix raises additional development capital to investigate OC459 for … – News-Medical.net


News-Medical.net

Atopix raises additional development capital to investigate OC459 for
News-Medical.net
Atopix Therapeutics Limited ("Atopix"), a UK biopharmaceutical company developing a novel class of medicines to treat atopic dermatitis and severe asthma, today announced that it has raised additional development capital to advance its lead product …

and more »

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