Tag: serum

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Elevated Serum IgE against MGL_1304 in Patients with Atopic Dermatitis and Cholinergic Urticaria.

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Elevated Serum IgE against MGL_1304 in Patients with Atopic Dermatitis and Cholinergic Urticaria.

Allergol Int. 2014 Jan 25;

Authors: Hiragun M, Hiragun T, Ishii K, Suzuki H, Tanaka A, Yanase Y, Mihara S, Haruta Y, Kohno N, Hide M

Abstract
Background: MGL_1304 secreted by Malassezia globosa is contained in human sweat and induces histamine release from basophils in patients with atopic dermatitis (AD) at a high positive rate. The aims of this study were to establish the enzyme-linked immunosorbent assay (ELISA) measuring specific immunoglobulins against MGL_1304 and to investigate the levels of these immunoglobulins in sera of patients with various allergic diseases. Methods: Purified MGL_1304 from human sweat (QRX) and recombinant MGL_1304 (rMGL_1304) were prepared for ELISA. To quantify the amount of MGL_1304-specific immunoglobulins, the standard serum was created by pooling sera of 20 patients with AD whose basophils released histamine in response to QRX. A monoclonal antibody which exhibited the highest neutralizing ability against QRX was established as Smith-2, and used as a capture antibody for the assay of QRX-specific IgE. A total of 156 subjects [normal controls (n = 23), AD (n = 63), cholinergic urticaria (CU) (n = 24), bronchial asthma (n = 32), and allergic rhinitis (n = 14)] were enrolled in this study. Results: ELISA methods to quantify the specific IgE, IgG and IgG4 against MGL_1304 in sera were successfully established. Levels of QRX-specific IgE in sera of patients with AD and CU were significantly higher than those of normal controls. Moreover, the levels of QRX-specific IgE and rMGL_1304-specific IgE in patients with AD were significantly correlated with their disease severities. Conclusions: These ELISA methods to quantify the specific immunoglobulins against MGL_1304 are easy and useful means to assess allergy to MGL_1304. MGL_1304 contained in sweat is an important antigen for patients with AD and CU.

PMID: 24457815 [PubMed – as supplied by publisher]

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Reference Intervals for Serum Immunoglobulins IgG, IgA, IgM and Complements C3 and C4 in Iranian Healthy Children.

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Reference Intervals for Serum Immunoglobulins IgG, IgA, IgM and Complements C3 and C4 in Iranian Healthy Children.

Iran J Public Health. 2012;41(7):59-63

Authors: Kardar G, Oraei M, Shahsavani M, Namdar Z, Kazemisefat G, Haghi Ashtiani M, Shams S, Pourpak Z, Moin M

Abstract
BACKGROUND: Determination of reference ranges of each serum protein in normal population of each country is required for studies and clinical interpretation. The aim of this study was defining reference range values of immunoglobulins and complement components in Iranian healthy children.
METHODS: This study was conducted from June 2003 to June 2006 in Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences. Serum levels of IgG, IgM, IgA, C3 and C4 in 800 Iranian healthy children from newborn to 18 years of age in four population were measured by nephelometry. Kolmogrov-Smirnov tests and Pearson correlation tests were used for analysis.
RESULTS: Our results mainly agree with previous reports, except for some discrepancy that might be due to the ethnic and geographic variety. There was a significant difference between two sexes only with IgA in the group of 1-3 months old, which was higher in male group and IgM in groups of 3-5, 6-8 and 9-11 years old that were higher in female groups. Mean of other serum immunoglobulins and complements was not significantly different between male and female groups.
CONCLUSION: These results can be considered as a local reference for use in laboratories, clinical interpretations, and research for Iranian children.

PMID: 23113211 [PubMed – in process]

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Proteome changes in auricular lymph nodes and serum after dermal sensitization to toluene diisocyanate in mice.

Proteome changes in auricular lymph nodes and serum after dermal sensitization to toluene diisocyanate in mice.

Proteomics. 2012 Oct 4;

Authors: Haenen S, Clynen E, De Vooght V, Schoofs L, Nemery B, Hoet PH, Vanoirbeek JA

Abstract
Some reactive chemicals, such as diisocyanates, are capable of initiating an allergic response, which can lead to occupational asthma after a latency period. Clinical symptoms such as cough, wheezing and dyspnea occur only late, making it difficult to intervene at an early stage. So far, most studies using proteomics in lung research have focused on comparisons of healthy vs. diseased subjects. Here, using two-dimensional difference gel electrophoresis (2D-DIGE), we explored proteome changes in the local draining lymph nodes and serum of mice dermally sensitized once or twice with TDI before asthma is induced. In the lymph nodes, we found 38 and 58 differentially expressed proteins after one and two treatments, respectively, between toluene diisocyanate-treated and vehicle-treated mice. In serum, 7 and 16 differentially expressed proteins were detected after one and two treatments, respectively. We identified 80-85% of the differentially expressed proteins by mass spectrometry. Among them, lymphocyte specific protein-1, coronin 1a and hemopexin were verified by Western blotting or ELISA in an independent group of mice. This study revealed alterations in the proteomes early during sensitization in a mouse model before the onset of chemical-induced asthma. If validated in humans, these changes could lead to earlier diagnosis of TDI-exposed workers.

PMID: 23038679 [PubMed – as supplied by publisher]

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Impact of asthmatic control status on serum cystatin C concentrations.

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Impact of asthmatic control status on serum cystatin C concentrations.

Clin Chem Lab Med. 2012 Aug;50(8):1367-71

Authors: Shigemura M, Konno S, Nasuhara Y, Shimizu C, Matsuno K, Nishimura M

Abstract
BACKGROUND: To determine whether cystatin C accurately reflects renal function in asthma, we investigated serum cystatin C concentrations in a large number of asthmatic patients by adjusting for several confounding factors that might affect serum cystatin C concentrations.
METHODS: A total of 126 asthmatic patients and 126 healthy volunteers, matched for age and gender, were studied.
RESULTS: Serum cystatin C concentrations in symptomatic subjects with asthma were significantly higher than in healthy controls (p < 0.001) and asymptomatic subjects with asthma (p = 0.007), whereas no significant difference was observed between healthy controls and asymptomatic subjects. In asthmatic subjects, serum cystatin C concentrations were not influenced by inhaled corticosteroid (ICS). However, serum cystatin C concentrations were significantly higher in subjects who were regularly treated by oral corticosteroid (OCS) (p = 0.001).
CONCLUSIONS: Serum cystatin C concentrations are elevated in asthmatic patients; particularly while symptomatic and/or taking OCS but not ICS. Serum cystatin C concentrations may not accurately reflect renal function in those patients.

PMID: 23035264 [PubMed – in process]

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Low Serum Adiponectin Levels Predict Future Asthma Risk in Women – Newswise (press release)

Low Serum Adiponectin Levels Predict Future Asthma Risk in Women
Newswise (press release)
Newswise — Low serum adiponectin levels predict an increased future risk for developing asthma in middle-aged women, particularly among smokers, according to a new study. “Adiposity is known to be related to asthma. Although a causal link between …

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[Lipid composition of cells and low-density lipoproteins in blood serum of humans and some vertebrates species].

[Lipid composition of cells and low-density lipoproteins in blood serum of humans and some vertebrates species].

Zh Evol Biokhim Fiziol. 2011 Sep-Oct;47(5):365-74

Authors:

Abstract
To investigate interaction of atherogenic low-density lipoproteins (LDL) with erythrocytic membrane, the content of lipid components in blood cells and serum LDL was studied in human in norm (donors) and in 12 species of vertebrates (the mammals non-predisposed to atherosclerosis – birds and fish). Lipid composition of blood cells and LDL was analyzed also in patients with pathologies: ischemic heart disease (IHD), bronchial asthma (BA), and chronic obstructive bronchitis (COB), and in 2 species of mammals predisposed to atherosclerosis, in whose blood LDL predominates. The content of lipids in cells and LDL of the studied vertebrates has been found to depend on their taxonomy and the clear trends both to an increase of the cholesterol content and to a decrease if the phosphatidylcholine level in patients, particu- larly with IHD, and on a rise of the ratio of the content of the more saturated sphingomyelin and cholesterol to the less saturated phosphatidylcholine from the lower to the higher organisms, including humans (donors). The highest levels of free cholesterol in blood cells, of total cholesterol in LDL, and of ration of the cholesterol/phosphatidylcholine content have been revealed in patients, especially with 1HB, and in the mammals predisposed to atherosclerosis, i. e., in representatives with predominance of blood LDL, unlike donors and the mammals resistant to atherosclerosis. The highest parameters of lipid components were determined in cells and LDL inhuman with IHD. The lipid LDL composition affects directly the composition and ratio of lipids in blood cells.

PMID: 22145317 [PubMed – in process]

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Severity Predictors in Eschar-Positive Scrub Typhus and Role of Serum Osteopontin.

Severity Predictors in Eschar-Positive Scrub Typhus and Role of Serum Osteopontin.

Am J Trop Med Hyg. 2011 Nov;85(5):924-930

Authors: Park SW, Lee CS, Lee CK, Kwak YG, Moon C, Kim BN, Kim ES, Kang JM, Oh MD

Abstract
Abstract. We prospectively evaluated severity predictors in terms of host, microorganism, and treatment factors in 153 eschar-positive scrub typhus patients. Severity was assessed with the Acute Physiology and Chronic Health Evaluation (APACHE) II score (< 10 versus ? 10) and predefined criteria of severe complications. Genotypes of Orientia tsutsugamushi were determined. Independent risk factors for severity (APACHE II score ? 10) were old age, diabetes mellitus, serum osteopontin > 100 ng/mL, and a group of underlying diseases (congestive heart failure, cerebrovascular disease, chronic liver disease, bronchial asthma, and chronic obstructive lung diseases). Anemia (? 10 g/dL) and C-reactive protein > 10 mg/dL were indicators of current severity. Neither the delay in antibiotics administration nor strain types (Boryong, Taguchi, or Kanda/Kawasaki) contributed to the severity. The risk factors for severe complications were similar. Serum osteopontin > 100 ng/mL had a negative predictive value of 96% for severe complications. This marker can be used to rule out severe disease status.

PMID: 22049051 [PubMed – as supplied by publisher]

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