Proteome changes in auricular lymph nodes and serum after dermal sensitization to toluene diisocyanate in mice.
Proteomics. 2012 Oct 4;
Authors: Haenen S, Clynen E, De Vooght V, Schoofs L, Nemery B, Hoet PH, Vanoirbeek JA
Abstract
Some reactive chemicals, such as diisocyanates, are capable of initiating an allergic response, which can lead to occupational asthma after a latency period. Clinical symptoms such as cough, wheezing and dyspnea occur only late, making it difficult to intervene at an early stage. So far, most studies using proteomics in lung research have focused on comparisons of healthy vs. diseased subjects. Here, using two-dimensional difference gel electrophoresis (2D-DIGE), we explored proteome changes in the local draining lymph nodes and serum of mice dermally sensitized once or twice with TDI before asthma is induced. In the lymph nodes, we found 38 and 58 differentially expressed proteins after one and two treatments, respectively, between toluene diisocyanate-treated and vehicle-treated mice. In serum, 7 and 16 differentially expressed proteins were detected after one and two treatments, respectively. We identified 80-85% of the differentially expressed proteins by mass spectrometry. Among them, lymphocyte specific protein-1, coronin 1a and hemopexin were verified by Western blotting or ELISA in an independent group of mice. This study revealed alterations in the proteomes early during sensitization in a mouse model before the onset of chemical-induced asthma. If validated in humans, these changes could lead to earlier diagnosis of TDI-exposed workers.
PMID: 23038679 [PubMed – as supplied by publisher]
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